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Sanger sequencing reaction mixtures included purified template DNA, a primer, DNA polymerase, and all four dNTPs. Each reaction also included a radiolabeled ddNTP version of just one of the four nucleotides. Only a small amount of each ddNTP was added, however, to ensure that a fraction of the total DNA fragments produced stopped at each occurrence of that base. As with previous methods, separating fragments via length and performing autoradiography allowed scientists to read the final sequence.，详情可参考服务器推荐
No silent fallback values，更多细节参见谷歌浏览器下载
（二）实际承运人，是指接受承运人委托或者转委托，实际履行本法第四十九条规定的全部或者部分承运人义务的人。